貌似巯基乙醇（2-mercaptoethanol）和碱性磷酸酶（alkaline phosphatase）不兼容

"The addition of 1% 2-mercaptoethanol caused the dissociation of alkaline phosphatase into two subunits of identical molecular weight, 75,000."

引自 Rat Liver Alkaline Phosphatase PURIFICATION AND PROPERTIES
Akiyuki Ohkubo, Neal Langerman , and Marshall M. Kaplan在JBC上发表的文章。

今天中午还为使用那个缓冲液犯愁，早早google一下就没这些麻烦事了。

另一条结果：

Chromatography on DEAE-Sephadex after the heating of the enzyme preparation at 37 degrees C and pH 5.5 in the presence of 2-mercaptoethanol was used to elimate the phosphatase contamination.

真险恶呀！